
ELK Biotechnology Biotinylated Human IgG1 Fc
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Product name: | Biotinylated Human IgG1 Fc |
Description: | Biotinylated Recombinant Human Immunoglobulin G1 Fc is produced by our Mammalian expression system and the target gene encoding Asp104-Lys330(Asp239Glu,Leu241Glu) is expressed with a Avi tag at the C-terminus. |
Expression Host: | Human Cells |
Accession: | P01857 |
Molecular weight: | 27.7 KDa |
Apparent molecular weight: | 33 KDa, reducing conditions |
Other names: | g gamma-1 chain C region; IGHG1 |
FORMULATION: | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Redissolve: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Delivery condition: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
Background: | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment (crystallizable fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. |
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