ELK Biotechnology Biotinylated Human Mesothelin (C-6His-Avi)
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| Product name: | Biotinylated Human Mesothelin (C-6His-Avi) |
| Description: | Biotinylated Recombinant Human Mesothelin is produced by our Mammalian expression system and the target gene encoding Glu296-Ser598 is expressed with a 6His, Avi tag at the C-terminus. |
| Expression Host: | Human Cells |
| Accession: | AAH09272.1 |
| Molecular weight: | 36.7 KDa |
| Apparent molecular weight: | 38-60 KDa, reducing conditions |
| Other names: | Megakaryocyte potentiating factor; mesothelin; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein; CAK1; MPF; MSLN; SMR; CAK1; CAK1 antigen |
| FORMULATION: | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin: | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| Biological activity: | Loaded Anti-Human Mesothelin mAb-Fc(Cat#NC078) on Protein A Biosensor, can bind Biotinylated Human Mesothelin-His-Avi(Cat#CY05) with an affinity constant of 10-3 nM as determined in BLI assay. |
| Redissolve: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Delivery condition: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Background: | Mesothelin is a cell surface glycoprotein whose expression is limited to mesothelial cells of the serosa (pleura, pericardium, and peritoneum) and epithelial cells of the trachea, tonsils, fallopian tube, and kidneys. Mesothelin plays an important role in cell survival, proliferation, migration, invasion, tumor progression, and resistance to chemotherapy. The overexpression of mesothelin can activate NF-κB and signal transducer and activator of transcription 3 (Stat3), inhibit apoptotic signaling and TNF-α-induced apoptosis, and accelerate the G1–S transition. Mesothelin is also found overexpressed in various cancers, including malignant mesothelioma, pancreatic or ovarian carcinoma, sarcomas and in some gastrointestinal or pulmonary carcinomas. As a result of its limited expression in normal tissues, mesothelin has been reported as an ideal tumor-associated marker for the development of targeted therapy. |
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