ELK Biotechnology Recombinant Human IFN gamma (E. coli)
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| Product name: | Recombinant Human IFN gamma (E. coli) |
| Description: | Recombinant Human Interferon Gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed. |
| Expression Host: | E.coli |
| Accession: | P01579 |
| Molecular weight: | 16.88 KDa |
| Apparent molecular weight: | 16 KDa, reducing conditions |
| Other names: | Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG |
| FORMULATION: | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, 5% Mannitol, 0.1% Tween80, 5% Trehalose, pH6.0. |
| Purity: | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin: | Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test. |
| Biological activity: | Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml. |
| Redissolve: | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage: | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Delivery condition: | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
| Background: | IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF. |
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