Thermo Fisher Scientific Acetyl-HMGB1 (Lys29) Polyclonal Antibody
Acetyl-HMGB1 (Lys29) 폴리클로날 항체로 인간, 생쥐, 랫트 반응성. Western blot, ICC/IF, ELISA에 사용 가능. 액상 형태로 0.47 mg/mL 농도, PBS/glycerol 버퍼에 보관. 세포막, 핵, 세포질 등 다양한 위치의 HMGB1 검출에 적합.
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Thermo Fisher Scientific Acetyl-HMGB1 (Lys29) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| ELISA | 1 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic acetylated peptide around K29 of human HMGB1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.47 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913988 |
Product Specific Information
Positive test controls include: 293T cells.
The target is usually found in the following locations:
- Cell membrane
- Chromosome
- Cytoplasm
- Endoplasmic reticulum-Golgi intermediate compartment
- Endosome
- Extracellular side
- Nucleus
- Peripheral membrane protein
- Secreted
Target Information
HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein.
Recent studies show that damaged or necrotic cells release HMGB1 into the extracellular space, where it functions as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:
- Two tandem-linked DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162)
- Negatively charged 30 aa C-terminal acidic tail region
Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The cytokine activity resides in the B box, while the A box interacts with transcription factors.
HMGB1 acts as an inflammatory mediator promoting monocyte migration and cytokine secretion, and facilitates T cell–dendritic cell interactions.
HMGB1 signals through receptors such as RAGE, TLR2, and TLR4, and is highly conserved across species.
It plays a key role in sterile and infection-associated inflammation and is implicated in trauma, sepsis, arthritis, and ischemia-reperfusion injury models.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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