Thermo Fisher Scientific Acetyl-HMGB1 (Lys29) Polyclonal Antibody

Thermo Fisher Scientific Acetyl-HMGB1 (Lys29) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Positive test controls include: 293T cells.
The target is usually found in the following locations:

• Cell membrane
• Chromosome
• Cytoplasm
• Endoplasmic reticulum-Golgi intermediate compartment
• Endosome
• Extracellular side
• Nucleus
• Peripheral membrane protein
• Secreted

Target Information

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA-binding chromosomal protein.
Recent studies show that damaged or necrotic cells release HMGB1 into the extracellular space, where it functions as a proinflammatory cytokine.

Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:

• Two tandem-linked DNA-binding domains (HMG box A: aa 9–79; box B: aa 89–162)
• Negatively charged 30 aa C-terminal acidic tail region

Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
The cytokine activity resides in the B box, while the A box interacts with transcription factors.
HMGB1 acts as an inflammatory mediator promoting monocyte migration and cytokine secretion, and facilitates T cell–dendritic cell interactions.

HMGB1 signals through receptors such as RAGE, TLR2, and TLR4, and is highly conserved across species.
It plays a key role in sterile and infection-associated inflammation and is implicated in trauma, sepsis, arthritis, and ischemia-reperfusion injury models.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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