Thermo Fisher Scientific KDM5B Polyclonal Antibody
Rabbit polyclonal antibody targeting KDM5B, validated for Western blot. Recognizes human KDM5B; predicted cross-reactivity with mouse and rat. Supplied as unconjugated liquid form, purified by antigen affinity chromatography. Ideal for epigenetic and c...
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1274–1498 of KDM5B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| RRID | AB_2553215 |
Product Specific Information
- Recommended positive control: MCF-7
- Predicted reactivity: Mouse (86%), Rat (90%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
KDM5B is a histone demethylase that demethylates Lys-4 of histone H3, playing a central role in the histone code. It does not demethylate histone H3 Lys-9 or Lys-27. KDM5B acts as a transcriptional corepressor for FOXG1B and PAX9, and promotes proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. Conversely, KDM5B may function as a tumor suppressor in melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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