Thermo Fisher Scientific alpha Actinin 4 Polyclonal Antibody
Western blot, IHC, ELISA, IP 등 다양한 응용에 적합한 alpha Actinin 4 폴리클로날 항체. Rabbit IgG로 제작되어 높은 특이성과 재현성을 제공. Human, Mouse, Rat 등 다양한 종에 반응하며, 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 140–190 of human Alpha Actinin 4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Alpha actinins belong to the spectrin gene superfamily, a group of cytoskeletal proteins including alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles across cell types.
In nonmuscle cells, it localizes along microfilament bundles and adherens-type junctions, linking actin to the membrane.
In muscle cells (skeletal, cardiac, smooth), it is found in the Z-disc and dense bodies, anchoring myofibrillar actin filaments.
This gene encodes a nonmuscle alpha actinin isoform concentrated in the cytoplasm and implicated in metastatic processes.
Mutations are associated with focal and segmental glomerulosclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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