Thermo Fisher Scientific IgD Monoclonal Antibody (IA6-2), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific IgD Monoclonal Antibody (IA6-2), NovaFluor Yellow 660

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

Product Specifications

Additional Formats

• Brilliant Violet 480
• Brilliant Violet 650
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-30S
• NovaFluor Blue 660-40S
• NovaFluor Blue 690
• NovaFluor Blue 725
• NovaFluor Blue 760
• NovaFluor Blue 800
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor UV 765
• NovaFluor Violet 690
• NovaFluor Violet 800
• NovaFluor Yellow 755
• NovaFluor Yellow 810
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). IgD is co-expressed on the surface of mature naive B cells with membrane IgM and also exists as a soluble form in blood serum. It associates with the B cell receptor and participates in signal transduction upon antigen binding.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated by flow cytometry on normal human peripheral blood cells. Use at 5 µL (0.2 µg)/test in a final volume of 100 µL. Cell number per test: 10⁵–10⁸ cells.

Compatibility Notes:
NovaFluor dyes are not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI).
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability analysis.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal staining with NovaFluor conjugates
• Add 5 µL per sample before combining antibody cocktails with cells
• For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells)

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for minimal spillover and spread.

Excitation: 552 nm
Emission: 663 nm
Laser: 561 nm (Yellow)

Target Information

IgD is a type I transmembrane protein co-expressed with IgM on mature naive B cells and also found in soluble form in serum. It interacts with the B cell receptor to mediate signal transduction upon antigen binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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