Thermo Fisher Scientific DnaJ Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

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Immunoprecipitation (IP)

-

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Product Specifications

Species Reactivity

Bacteria

Published species

Bacteria

Host/Isotype

Mouse / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues S(13) K T A E E R E I R K A Y K(27) of E. coli DnaJ.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites, PBS

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_325969

Product Specific Information

PA3-018 detects DnaJ from bacteria.

PA3-018 has been successfully used in Western blot procedures. By Western blot, this antibody detects a single ~40 kDa band representing DnaJ from E. coli extract.

The PA3-018 immunogen is a synthetic peptide corresponding to residues S(13) K T A E E R E I R K A Y K(27) of E. coli DnaJ.

Target Information

In prokaryotes, the heat shock response is characterized by the induction of several proteins including DnaJ, DnaK, GroEL and GroES. DnaK is a member of the HSP70 family, and like other members, exhibits ATPase activity which facilitates the release of bound proteins and disaggregation of protein complexes within the cell. Members of this protein group serve as molecular chaperones. Levels of DnaJ and DnaK are closely linked and, in E. coli, are constituents of a single operon. DnaJ regulates DnaK in several different systems. Relative to DnaK, the level of DnaJ synthesis is quite low. Studies have shown that DnaJ and DnaK, together with GrpE, cooperatively interact with firefly luciferase as it unfolds during thermal denaturation allowing renaturation of the protein upon return to normal temperatures. Also, DnaK together with DnaJ stimulates the binding of RepA initiator protein to the origin of P1 phage in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.