Thermo Fisher Scientific DnaJ Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA3-018 detects DnaJ from bacteria.
It has been successfully used in Western blot procedures.
By Western blot, this antibody detects a single ~40 kDa band representing DnaJ from E. coli extract.
The immunogen is a synthetic peptide corresponding to residues S(13) K T A E E R E I R K A Y K(27) of E. coli DnaJ.

Target Information

In prokaryotes, the heat shock response is characterized by the induction of several proteins including DnaJ, DnaK, GroEL, and GroES.
DnaK, a member of the HSP70 family, exhibits ATPase activity that aids in the release of bound proteins and disaggregation of complexes within the cell.
These proteins act as molecular chaperones. Levels of DnaJ and DnaK are closely linked and, in E. coli, are constituents of a single operon.
DnaJ regulates DnaK in several systems, and its synthesis level is relatively low compared to DnaK.
DnaJ and DnaK, together with GrpE, cooperatively assist in the refolding of firefly luciferase following thermal denaturation.
Additionally, DnaK and DnaJ stimulate the binding of RepA initiator protein to the origin of P1 phage in vitro.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.