Thermo Fisher Scientific Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), APC, eBioscience

Thermo Fisher Scientific Ly-49D Monoclonal Antibody (eBio4E5 (4E5)), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
• Publications: View 4 publications

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

The monoclonal antibody eBio4E5 (4E5) reacts with Ly-49D, an activating member of the Ly-49 family of NK cell receptors.
This family of receptors forms homodimers recognizing MHC Class I molecules.
Activating Ly-49 receptors (Ly-49D and Ly-49H) lack ITIM motifs but associate with DAP12, which contains ITAM motifs for positive signaling.

Expression of Ly-49D varies by mouse strain:

• Positive: C57BL/6, CB6F1, SJL
• Negative: BALB/c, DBA/2, AKR, CBA/J
• In 129/J mice, eBio4E5 also recognizes Ly-49O, Ly-49V, and Ly-49R.

Reported functional (activating) activity.

Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤ 0.5 µg/test (in 100 µL final volume).
Cell count range: 10⁵–10⁸ cells/test.
Careful titration recommended for optimal performance.
Excitation: 633–647 nm; Emission: 660 nm; Laser: Red Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ly-49D is an activating NK cell receptor that recognizes MHC Class I molecules.
Unlike inhibitory Ly-49 receptors, Ly-49D associates with DAP12 for activating signal transduction.
Expression is strain-dependent and may overlap with other Ly-49 family members on the same NK cells.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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