Thermo Fisher Scientific NFkB p65 Polyclonal Antibody
NFkB p65 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되었으며, 고순도 및 재현성 높은 결과 제공.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | 1 publication |
| Immunohistochemistry (IHC) | – | 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | – |
| Immunoprecipitation (IP) | Assay-dependent | – |
| ChIP assay (ChIP) | Assay-dependent | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–260 of Human NFkB p65 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.49 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545093 |
Product Specific Information
- Recommended positive controls: 293T, HeLa, HepG2, K562, THP-1, HL-60, human NFkB p65-transfected 293T
- Predicted reactivity: Mouse (96%), Rat (99%), Pig (98%), Chimpanzee (99%), Bovine (98%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Nuclear factor kB p65 (NF-kB p65) is encoded by the RELA gene located on chromosome 11 in humans.
NF-kB p65, also known as RelA, belongs to the Rel family of proteins and forms a heterodimer with p50 or p52 subunits of NF-kB (Nuclear factor kappa-light-chain-enhancer of activated B cells).
Upon TNF-α binding to its receptor, IKK is phosphorylated, leading to phosphorylation and proteasomal degradation of IκB. This process releases NF-kB p65 to translocate into the nucleus, where it regulates transcription of target genes, including the immunoglobulin κ (kappa) gene in mature B-lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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