Thermo Fisher Scientific MagniSort Mouse CD49b Positive Selection Kit

Invitrogen™ MagniSort™ Mouse CD49b Positive Selection Kit

The MagniSort™ Mouse CD49b Positive Selection Kit is designed for the magnetic separation of CD49b⁺ cells by positive selection. It has been optimized for isolating CD49b⁺ cells from mouse spleens or lymph nodes using a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads. CD49b⁺ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, undesired cells can be separated from CD49b⁺ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136.
For mouse strains that do not express NK1.1 antigen (e.g., BALB/c, SJL, AKR, CBA, C3H), Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.

Reported Application

• Magnetic Cell Separation

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