Thermo Fisher Scientific IDH3A Polyclonal Antibody
Thermo Fisher Scientific의 IDH3A 폴리클로날 항체는 Western blot 및 IHC(P) 실험에 적합하며, 인간, 마우스, 랫트에 반응합니다. 항원 친화 크로마토그래피로 정제된 액상 항체로, 0.63 mg/mL 농도를 가지며 단기 4°C, 장기 -20°C 보관이 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 129–366 of Human IDH3A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.63 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2547543 |
Product Specific Information
- Recommended positive controls: Raji, mouse brain, rat brain
- Predicted reactivity: Mouse (98%), Rat (97%), Zebrafish (88%), Xenopus laevis (88%), Chicken (92%), Rhesus Monkey (99%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one utilizing NAD(+) and the other NADP(+) as electron acceptors.
Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent enzymes localized to the mitochondrial matrix, and two NADP(+)-dependent enzymes, one mitochondrial and one cytosolic.
NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated, rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer composed of two alpha, one beta, and one gamma subunit. The protein described here is the alpha subunit of one NAD(+)-dependent isozyme.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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