Invitrogen™

TaqMan™ Cells-to-CT™ Express Lysis Reagents

This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in자세히 알아보기

This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit. The reagents include Express Lysis Buffer and Express ezDNase. They are ideal for preparing RNA from cultured mammalian cells for gene expression analysis. The lysis procedure simultaneously prepares cell lysates for RT-qPCR and removes genomic DNA in only five minutes. The lysis step is simple to automate with robotic platforms for high-throughput processing of 96- or 384-well plates because it takes place entirely at room temperature. The procedure is also economical as there are only a few pipetting steps and, for cells grown in 96- or 384-well plates, no sample transfers.

Features of the TaqMan Cells-to-CT Express Lysis Reagents include:
• Extraordinary ease and speed—96 samples ready for RT-qPCR in as little as 5 minutes
• No tedious RNA purification—no columns, heating, or centrifugation
• Environmentally friendly—the kit is REACH Compliant, has fewer components, and requires fewer pipetting steps, saving plastics costs
• Right sized kits for automation—available 2500 reaction size includes both lysis and RT components

Optimized chemistry with no need for stop solution
Gene expression analysis from direct cell lysis is made simple with our TaqMan Cells-to-CT Express Kit. With only one step for cell lysis and no need for the addition of a Stop Solution, our Express technology enables a rapid workflow with sensitive, accurate, and reproducible results. 10–100,000 cells are lysed using the Express Lysis Solution. The optional inclusion of ezDNase to the Express Lysis Solution enables removal of genomic DNA during cell lysis. Following a 5-minute incubation at room temperature, the lysate is used directly for reverse transcription and qPCR analysis.

사양

검출 방법Primer-probe

형태Liquid

형식Kit

GC-Rich PCR PerformanceHigh

PCR 방법2-step RT-qPCR

반응 속도Fast

그린 기능Less hazardous, less waste

고처리량 호환성Yes

키트 유형Direct Cell Lysis Reagents

라벨 또는 염료ROX

수량2500 reactions

유통 기한1 Year

배송 조건Frozen, Dry Ice

타겟cDNA

용도(애플리케이션)Gene Expression

분리 기술Direct cell lysis

반응 수2500 Reactions

정제 타겟RNA

샘플 종류Cultured mammalian cells

Unit SizeEach