Thermo Fisher Scientific PYCRL Monoclonal Antibody (OTI1B12), TrueMAB
PYCRL 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry에 적합. 인간 및 랫트 반응성. 동결건조 형태로 제공되며, PBS 버퍼에 8% 트레할로스 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B12 |
| Immunogen | Full-length human recombinant PYCRL protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PYCRL encodes a cytoplasmic enzyme belonging to the pyrroline-5-carboxylate reductase family, which catalyzes the final step in proline biosynthesis by converting pyrroline-5-carboxylate to proline.
Glutamate and ornithine act as precursors in this biosynthetic pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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