Thermo Fisher Scientific GARP Monoclonal Antibody (YGIC86), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.125 µg/test
• Publications: View 6 publications

Product Specifications

Additional Available Formats

• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Product Specific Information

Description:
The YGIC86 monoclonal antibody reacts with mouse Glycoprotein A Repetitions Predominant (GARP, also known as Lrrc32 or Garpin). GARP is an approximately 80 kDa glycoprotein expressed on the cell surface. It is found in placenta, lung, kidney, heart, ovary, liver, skeletal muscle, and pancreas, and on megakaryocytes, platelets, and activated regulatory T (Treg) cells. GARP expression on Treg cells is critical for their suppressive function and may act as a receptor for LAP/TGF beta.

When analyzing GARP expression on platelets, use the Foxp3 Staining Buffer Set (Product # 00-5523) to fix platelets before staining. Fixation/permeabilization is not necessary for Treg cell staining.

YGIC86 does not cross-react with rat GARP.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on stimulated mouse splenocytes. Use ≤0.125 µg/test (100 µL final volume). Adjust cell number empirically (10⁵–10⁸ cells/test). Titrate for optimal performance.

Fluorescence Characteristics:

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

GARP (leucine rich repeat containing 32) is a type I membrane protein with 22 leucine-rich repeats and a short cytoplasmic region. Identified via gene expression profiling of Treg and Th cells after TCR stimulation, GARP is expressed in CD4⁺, CD25⁺, FoxP3⁺ Treg cells, serving as a potential surface marker for Tregs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지