Thermo Fisher Scientific ITCH Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human ITCH. Recombinant protein control fragment (Product #RP-104867). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ITCH,uniProtId:Q96J02-1,ncbiNodeId:9606,antigenRange:197-273,antigenLength:903,antigenImageFileName:PA5-65539_ITCH_Q96J02-1_Rabbit.svg,antigenImageFileNamePDP:PA5-65539_ITCH_Q96J02-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2664412

Product Specific Information

Immunogen sequence: GFTGASQNDDG SRSKDETRVS TNGSDDPEDA GAGENRRVSG NNSPSLSNGG FKPSRPPRPS RPPPPTPRRP ASVNGS

Highest antigen sequence identity to the following orthologs - mouse 78%, rat 82%.

Target Information

Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.