Thermo Fisher Scientific SF1 Monoclonal Antibody (2E12)
SF1 단백질을 인식하는 Thermo Fisher Scientific의 2E12 모노클로날 항체로, Western blot 및 ELISA에 적합합니다. 인간 및 생쥐 반응성을 가지며 비결합형 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific SF1 Monoclonal Antibody (2E12)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E12 |
| Immunogen | SF1 (NP_004621, 1–110 a.a) partial recombinant protein with GST tag (MW of GST tag alone: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Storage Buffer | Ascites |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
MATGANATPL DFPSKKRKRS RWNQDTMEQK TVIPGMPTVI PPGLTREQER AYIVQLQIED LTRKLRTGDL GIPPNPEDRS PSPEPIYNSE GKRLNTREFR TRKKLEEERH
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for early spliceosome assembly.
It also plays roles in nuclear pre-mRNA retention and transcriptional repression.
The protein contains:
- N-terminal U2AF ligand motif
- Central hnRNP K homology motif and quaking 2 region (binds key branch-site adenosine)
- Zinc knuckle domain
- C-terminal proline-rich domain
Alternative splicing results in multiple transcript variants.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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