Thermo Fisher Scientific CPSF30 Polyclonal Antibody

Thermo Fisher Scientific CPSF30 Polyclonal Antibody

Applications and Tested Dilution

• Western Blot (WB): 1:2,000–1:10,000
• Immunoprecipitation (IP): 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• 권장 유효기간: 수령일로부터 1년
• 100% 서열 동일성 기준으로 Rat, X. laevis, X. tropicalis, Bovine에도 반응할 것으로 예측됨

Target Information

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4 (CPSF4), an essential component of the 3′ end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with CPSF4, 30kD subunit. Binding of NS1 to the 30 kDa protein prevents CPSF binding to RNA substrate and inhibits 3′ end cleavage and polyadenylation of host pre-mRNAs, selectively inhibiting nuclear export of cellular mRNAs. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.