Thermo Fisher Scientific CD80 (B7-1) Monoclonal Antibody (16-10A1), Super Bright 436, eBioscience
마우스 CD80(B7-1)을 인식하는 16-10A1 단클론 항체로, Super Bright 436 형광 표지. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 자외선(405 nm) 레이저로 여기됨. 0.06 µg/test 권장, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.06 µg/test | View 42 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Mouse, Pig |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16-10A1 |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413/431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2716995 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- Biotin
- Brilliant UV 615
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 605
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine5
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
The 16-10A1 monoclonal antibody reacts with mouse CD80 (B7-1), a 55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, and activated B cells, as well as activated T cells. It binds to T cell surface antigens CD28 and CD152 (CTLA-4), playing a crucial role in T-B cell communication and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested by flow cytometric analysis of stimulated mouse splenocytes (≤ 0.06 µg/test)
- Recommended Conditions: Final volume 100 µL, cell number 10⁵–10⁸ cells/test; titrate for optimal performance
Fluorochrome Information:
Super Bright 436 is excited with the violet laser (405 nm) and emits at 436 nm. Recommended detection with a 450/50 bandpass filter or equivalent.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152). Both are expressed on antigen-presenting cells and are essential for T cell activation. CD80 is rapidly induced on activated B cells, EBV-transformed and Burkitt lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It regulates T lymphocytes via binding to CD28 and CTLA4.
Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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