Thermo Fisher Scientific eIF3E Polyclonal Antibody
Thermo Fisher Scientific의 eIF3E Polyclonal Antibody는 인간 및 마우스 반응성을 가지며, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 액상 항체로, 4°C에서 보관하며 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 50 and 100 of human Eukaryotic Translation Initiation Factor 3, Subunit E |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, and Orangutan
Target Information
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex also plays a role in disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Required for nonsense-mediated mRNA decay (NMD); may act with UPF2 to divert mRNAs from translation to the NMD pathway.
May interact with MCM7 and EPAS1 and regulate proteasome-mediated degradation of these proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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