Thermo Fisher Scientific eIF3E Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
• Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, and Orangutan

Target Information

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis.
The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi, and eIF-5 to form the 43S preinitiation complex (43S PIC).
It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition.
The eIF-3 complex also plays a role in disassembly and recycling of post-termination ribosomal complexes and prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Required for nonsense-mediated mRNA decay (NMD); may act with UPF2 to divert mRNAs from translation to the NMD pathway.
May interact with MCM7 and EPAS1 and regulate proteasome-mediated degradation of these proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.