Thermo Fisher Scientific Phospho-ROCK1 (Thr455, Ser456) Polyclonal Antibody
Phospho-ROCK1 (Thr455, Ser456) 항체로 세포 분화 및 이동 연구에 적합. IHC, ICC, ELISA 등 다양한 응용 가능. Rabbit Polyclonal 형태로 1 mg/mL 농도. ROCK1 신호전달 및 apoptosis 관련 연구용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human ROCK1 around the phosphorylation site of Thr455 and Ser456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
Target Information
ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been identified as a Caspase-3 substrate. It contributes to myosin light chain (MLC) activation through direct phosphorylation of MLC and inhibition of MLC phosphatase. This process increases cellular contractility, which is essential for apoptotic membrane blebbing.
ROCK1 consists of an amino-terminal kinase domain and a cysteine/histidine-rich C-terminal inhibitory domain within a pleckstrin-homology region, connected by a variable region containing the Rho-binding domain. During apoptosis, caspase-3 cleaves ROCK1 at a conserved DETD1113/G sequence, removing the inhibitory domain and resulting in constitutive kinase activity. This cleavage induces MLC phosphorylation and membrane blebbing, key events in the execution phase of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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