Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 700
Alexa Fluor 700 형광을 결합한 염소 유래 항-마우스 IgG(H+L) 2차 항체로, WB, ICC/IF, Flow Cytometry에 사용 가능. 인간 IgG에 대한 교차흡착 처리로 높은 특이성과 낮은 배경 신호 제공. 근적외선 형광 검출 및 멀티플렉싱 분석에 최적화.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 700
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2535707 |
| Target | IgG |
| Cross Adsorption | Against human IgG and human serum prior to conjugation |
| Antibody Form | Whole Antibody |
Product Specific Information
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments.
It can be used for multi-color and multiplexing detection with other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths.
Also suitable for immunofluorescent and fluorescent imaging applications using appropriate excitation and detection capabilities.
Product will be shipped at room temperature.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
Useful for detection, sorting, or purification of target proteins.
Secondary antibodies enhance versatility and sensitivity via signal amplification, as multiple secondary antibodies can bind to one primary antibody.
Typically generated by immunizing host animals with pooled mouse immunoglobulins, followed by purification and optional modification (e.g., immunoaffinity chromatography, fragmentation, conjugation).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Conjugates
- Agarose
- Alexa Fluor 350, 405, 430, 488, 514, 532, 546, 555, 568, 594, 633, 635, 647, 660, 680, 750, 790
- AP, APC, APC-Alexa Fluor 750
- Biotin, Biotin-XX
- BODIPY FL, Cyanine3, Cyanine5
- DyLight 350, 405, 488, 550, 594, 633, 650, 680, 755, 800
- FITC, HRP, Pacific Blue, PE, PE-Alexa Fluor 610
- Rhodamine, Rhodamine Red-X, Texas Red-X, TRITC
- Custom conjugation available upon request
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