Thermo Fisher Scientific eIF4G1/eIF4GI Polyclonal Antibody
eIF4G1/eIF4GI 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용에 적합. 비결합(Unconjugated) 형태의 액상 제품이며, 4°C에서 보관. FFPE 조직의 IHC 시 citrate buffer(pH 6.0) 전처리 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 525 and 575 of human eukaryotic translation initiation factor 4 gamma, 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene is a component of the EIF4F complex, which participates in mRNA cap recognition, ATP-dependent unwinding of 5'-terminal secondary structures, and recruitment of mRNA to the ribosome.
Alternative splicing results in five transcript variants encoding four distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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