Thermo Fisher Scientific THOC5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein fragment corresponding to a region within amino acids 308 and 641 of Human THOC5 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:THOC5,uniProtId:Q13769-1,ncbiNodeId:9606,antigenRange:308-641,antigenLength:683,antigenImageFileName:PA5-31643_THOC5_Q13769-1_Rabbit.svg,antigenImageFileNamePDP:PA5-31643_THOC5_Q13769-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2549116

Product Specific Information

Recommended positive controls: 293T, A431, HeLa.

Predicted reactivity: Human (99%), Mouse (94%), Rat (94%), Chicken (84%), Chimpanzee (100%), Bovine (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Component the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. The TREX complex is essential for the export of Kaposis sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction beteen KSHV ORF57 protein and THOC4. UAP56 functions as a bridge between THOC4 and the THO complex. THOC5 in conjunction with THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA. May be involved in the differentiation of granulocytes and adipocytes.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.