Thermo Fisher Scientific RCH1/KPNA2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:10,000–1:25,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:1,000–1:5,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt.
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

The import of proteins into the nucleus involves two steps:

1. Energy-independent docking of the protein to the nuclear envelope.
2. Energy-dependent translocation through the nuclear pore complex.

Imported proteins require a nuclear localization sequence (NLS) consisting of short regions of basic amino acids. KPNA2 interacts with the NLSs of DNA helicase Q1 and SV40 T antigen, and may be involved in nuclear transport of proteins. It may also play a role in VJ recombination.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.