Thermo Fisher Scientific Tramadol Polyclonal Antibody

Applications

• ELISA (ELISA)
  Tested Dilution: 5–10 µg/mL

Product Specifications

Product Specific Information

• Working Concentration: 2.5 µg/mL
• Sensitivity: 10 ng/mL Tramadol hydrochloride produces 78% inhibition in a competitive ELISA employing Tramadol polyclonal antibody.

Target Information

Tramadol is a narcotic analgesic used for moderate to severe pain and may be habit-forming. Tramadol and its O-desmethyl metabolite (M1) are selective, weak OP3-receptor agonists.
Opiate receptors are coupled with G-proteins that regulate synaptic transmission by activating effector proteins such as adenylate cyclase and cAMP. Opioids decrease intracellular cAMP by inhibiting adenylate cyclase, reducing the release of nociceptive neurotransmitters (substance P, GABA, dopamine, acetylcholine, noradrenaline).
Analgesic properties are attributed to inhibition of norepinephrine and serotonin reuptake in the CNS. The (+) enantiomer shows higher affinity for OP3 receptor and enhances serotonin release, while the (-) enantiomer preferentially inhibits norepinephrine reuptake via α2-adrenergic receptor stimulation.
Source: PubChem, CID=33741

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.