Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI2B11), TrueMAB
인간 CD80 단백질을 인식하는 Mouse IgG2a 단클론 항체로 WB, IHC, ICC/IF에 사용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 연구용으로 T세포 활성화 및 면역조절 관련 연구에 적합.
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Thermo Fisher Scientific CD80 Monoclonal Antibody (OTI2B11), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B11 |
| Immunogen | Full-length human recombinant protein of CD80 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD80 (B7-1) and CD86 (B7-2) are ligands for the T cell costimulatory molecule CD28 and inhibitory receptor CTLA-4 (CD152).
Both are expressed on antigen-presenting cells and play essential roles in T cell activation.
CD80 is induced on activated B cells, EBV-transformed B cell lines, Burkitt’s lymphoma cell lines, follicular lymphoma cells, T cells, and monocytes.
It provides regulatory signals to T lymphocytes via interaction with CD28 and CTLA4.
CD80 dysfunction is associated with diseases such as gallbladder squamous cell carcinoma and myocarditis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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