Invitrogen™

Retic Lysate IVT™ Kit

The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized자세히 알아보기

The Retic Lysate IVT™ Kit efficiently translates _in vitro_-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs. The optimized lysate results in high protein yield, biological activity, and consistent performance. Advantages of the Retic Lysate IVT™ Kit:

• Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs
• Accommodates large volume of template mRNA

How it works
The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA. Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers. These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences. A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon. The Retic Lysate IVT™ Kit was tested using Kozak`s constructs and it demonstrated high fidelity for initiating at the proper AUG.

Reference
1. Kozak, M. (1990) Evaluation of the fidelity of initiation of translation in reticulocyte lysates from commercial sources. Nucleic Acids Res 18:2828.

사양

형식Kit

발현 단백질 유형Water Soluble Protein

발현 메커니즘Cell-Free Expression

발현 시스템Cell-Free

용도(애플리케이션)In Vitro Transcription

반응 수60 Reactions

제품라인Ambion™

제품 유형Retic Lysate

수량60 Reactions

배송 조건Dry Ice

Unit SizeEach