Thermo Fisher Scientific cAMP-Screen Direct Cyclic AMP Immunoassay System, 192 assays (2 x 96)

Applied Biosystems™ cAMP-Screen Direct™ Cyclic AMP Immunoassay System

The cAMP-Screen Direct™ 96-well Cyclic AMP Immunoassay System enables ultrasensitive determination of cyclic AMP (cAMP) levels in cell lysates. This competitive chemiluminescent immunoassay offers flexibility for both manual and automated high-throughput processing.

The assay utilizes a chemiluminescent CSPD™ substrate with Sapphire-II™ enhancer, triggered by a cAMP–alkaline phosphatase (cAMP-AP) conjugate. The ready-to-use substrate/enhancer formulation provides a sustained glow signal from 30 minutes after addition, allowing extended read times.

주요 특징

• 상용화된 cAMP 어세이 중 최고 수준의 민감도
• 수 시간 동안 안정적인 발광 신호 유지
• 세포를 직접 플레이트에서 배양 가능
• 다양한 수용체 활성화 연구에 활용 가능

High Sensitivity and Extended Glow Time

This chemiluminescent assay provides the highest sensitivity among commercially available cAMP assays, detecting as few as 60 femtomoles of cAMP. It covers a wide dynamic range from 0.06 to 6,000 picomoles, without dilution or acetylation.
Intra-assay precision for duplicates is typically ≤5%. Once the glow signal is achieved, plates can be read for hours with minimal signal decay. The assay shows exceptionally low cross-reactivity with other adenosine-containing or cyclic nucleotides.

For Receptor Activation Studies

Designed for quantifying cellular cAMP in receptor activation assays, this system is suitable for:

• Established cell lines with endogenous or exogenous receptors
• Primary cell cultures and tissues treated with ligands
• Receptor characterization and orphan receptor ligand identification
• High-throughput screening of compounds affecting signal transduction

Simple Protocol — No Lysate Transfer Required

Cells are seeded, cultured, and treated directly in the assay plate. Lysates are prepared and incubated with cAMP-AP conjugate and anti-cAMP antibody in the coated microplate.
In samples lacking cAMP, high signal is produced; increasing cAMP concentration reduces signal proportionally. After washing, the chemiluminescent substrate is added, and the glow is measured using a luminometer.

This system eliminates the need to transfer lysates to separate plates, improving precision and reducing handling steps. The precoated microplates have a clear bottom for cell monitoring and support various cell types.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

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