Takara AAV purification kits

AAV vectors have emerged as highly effective gene delivery tools for therapeutic research, in part due to the ability to target specific cell/tissue types with a variety of recombinant AAV serotypes (Büning 2019). Generating AAV vectors with good efficacy requires a method that yields vectors of high purity. However, many currently available schemas are serotype dependent, requiring separate method development/optimization for each vector. Click More to see how our kits provide a solution.

AAV vectors have emerged as highly effective gene delivery tools for therapeutic research, in part due to the ability to target specific cell/tissue types with a variety of recombinant AAV serotypes (Büning 2019). Generating AAV vectors with good efficacy requires a method that yields vectors of high purity. However, many currently available schemas are serotype dependent, requiring separate method development/optimization for each vector.

Our All Serotypes kits solve this issue through the use of a simple precipitation and filtration protocol that can purify AAV particles with any capsid (i.e., from any serotype).

AAVpro Cell & Sup. Purification Kit Maxi (All Serotypes) allows the researcher to purify virus particles from both AAV-producing cells and the culture supernatant, providing higher virus yields. Each kit/pack contains sufficient reagents for four virus preps, each from a culture size of five T225 flasks. See table below for additional culture volume information. This kit contains:

• AAVpro Cell & Sup. Extraction/Concentration Pack Maxi (All Serotypes) allows researchers to extract virus particles from both AAV-producing cells and the culture supernatant, providing higher virus yields. Each pack contains sufficient reagents to prepare four preps of AAV particle extracts, each from a culture size of five T225 flasks. AAV viral particles can be further purified using the AAVpro Purification Pack Maxi (All Serotypes) (Cat. # 6678).
• AAVpro Purification Pack Maxi (All Serotypes) allows researchers to purify four virus preps, each from a culture size of five T225 flasks of AAV-producing cells and culture supernatant.

The AAVpro Purification Kit Maxi (All Serotypes) allows the researcher to purify four virus preps, each from a culture size of five T225 flasks. See table below for additional culture volume information.

Alternatively, the AAVpro Purification Kit Midi (All Serotypes) version of the kit provides four preps, each from a culture size of one T225 flask (or four 10-cm dishes). See table below for additional culture volume information.

The AAVpro Purification Kit (AAV2) is serotype dependent, and thus restricted to AAV2 since the protocol uses affinity-based purification. With this kit, you can purify two AAV2 preps, each from a culture size of five T225 flasks. See table below for additional culture volume information.

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