Thermo Fisher Scientific PRDX5 Monoclonal Antibody (3F11)
PRDX5 단일클론 항체(3F11)는 인간 Peroxiredoxin V 단백질을 인식하며 WB, ICC/IF, Flow, ELISA, IP 등에 사용 가능. HeLa 세포를 양성 대조군으로 권장. 비결합형 액상 형태로 -20°C 보관. 연구용으로 세포 증식 및 산화환원 관련 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 3 publications |
| Immunocytochemistry (ICC/IF) | 20 µL/mL | – |
| Flow Cytometry (Flow) | 1:20 | – |
| ELISA | Assay-dependent | – |
| Immunoprecipitation (IP) | 1–2 µL | – |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F11 |
| Immunogen | Purified recombinant human fragment protein (without mitochondrial leader sequence) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2168629 |
Product Specific Information
A suggested positive control for this product is HeLa cells.
Target Information
Peroxiredoxin (Prx) is a growing peroxidase family involved in cell proliferation, differentiation, and apoptosis. Mammalian Prx includes six members grouped into typical 2-Cys, atypical 2-Cys, and 1-Cys Prx types.
Except for Prx VI (1-Cys subgroup), the other five 2-Cys Prx isotypes exhibit thioredoxin-dependent peroxidase (TPx) activity, utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system.
Mammalian Prxs (20–30 kDa) vary in subcellular localization: Prx I, II, and VI in cytosol; Prx III in mitochondria; Prx IV in ER and secretion; Prx V distributed across peroxisome, mitochondria, and cytosol.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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