Thermo Fisher Scientific Prohibitin Monoclonal Antibody (PHB, 3194)
인간 Prohibitin 단백질을 인식하는 Mouse monoclonal antibody로, WB, IHC, ICC 등 다양한 응용에 적합합니다. Recombinant human PHB fragment(aa167-261)을 면역원으로 제작되었으며, 고순도의 Protein A/G 정제 제품입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PHB, 3194 |
| Immunogen | Recombinant human PHB protein fragment (aa167–261) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Prohibitin is encoded by an evolutionarily conserved gene that is ubiquitously expressed. It functions as a negative regulator of cell proliferation and may act as a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts differing in the length of their 3′ untranslated region. The longer transcript is more abundant in proliferating tissues and cells, suggesting a regulatory role for the extended 3′ UTR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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