Thermo Fisher Scientific SUPT3H Monoclonal Antibody (1A6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

1A6

Immunogen

SUPT3H (AAH50384, 1 a.a. approximately 328 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SPT3,uniProtId:O75486-1,ncbiNodeId:9606,antigenRange:1-317,antigenLength:317,antigenImageFileName:H00008464-M01A_SPT3_O75486-1_House_mouse.svg,antigenImageFileNamePDP:H00008464-M01A_SPT3_O75486-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Storage buffer

ascites

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MVVFGTTFFN FDSFIGKTDS IEIIGKSVFP YCYHNILPLL NDSGRYSLGD ARRPLHETAV LVEDVVHTQL INLLQQAAEV SQLRGARVIT PEDLLFLMRK DKKKLRRLLK YMFIRDYKSK IVKGIDEDDL LEDKLSGSNN ANKRQKIAQD FLNSIDQTGE LLAMFEDDEI DEVKQERMER AERQTRIMDS AQYAEFCESR QLSFSKKASK FRDWLDCSSM EIKPNVVAME ILAYLAYETV AQLVDLALLV RQDMVTKAGD PFSHAISATF IQYHNSAEST AACGVEAHSD AIQPCHIREA IRRYSHRIGP LSPFTNAYRR NGMAFLAC

Target Information

The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.