Thermo Fisher Scientific I-Kappa-B Kinase b (IKKb) Polyclonal Antibody
IKKβ 단백질을 인식하는 Thermo Fisher Scientific의 다클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 시료에 반응하며, 항원 친화 크로마토그래피로 정제된 액상 형태입니다. NF-κB 신호전달 연구용으로 이상적입니다.
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Thermo Fisher Scientific I-Kappa-B Kinase β (IKKβ) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full-length human IKKβ recombinant protein (UniProt ID: O14920-1, Range: 1–756) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C and avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Recombinant protein
- Cellular location: Nuclear
Target Information
IKKβ (Inhibitor of nuclear factor kappa-B kinase subunit beta) is a serine kinase essential for NF-κB signaling activation. Phosphorylation of I-kappa-B proteins by IKK kinases, including IKKβ, targets them for ubiquitin-mediated degradation, enabling NF-κB activation. IKKβ functions within the canonical IKK complex, phosphorylating inhibitors of NF-κB on key serine residues, leading to their polyubiquitination and proteasomal degradation. Activated NF-κB then translocates to the nucleus to regulate genes involved in immune response, growth control, and apoptosis protection. Mutations in the IKKβ gene are associated with Immunodeficiency 15 (IMD15).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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