Thermo Fisher Scientific c-Mpl Recombinant Rabbit Monoclonal Antibody (ARC2257)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC2257

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 536-635 of human TPO-R/CD110/c-Mpl (P40238) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:c-Mpl,uniProtId:P40238-1,ncbiNodeId:9606,antigenRange:536-635,antigenLength:635,antigenImageFileName:MA5-37967_c-Mpl_P40238-1_Rabbit.svg,antigenImageFileNamePDP:MA5-37967_c-Mpl_P40238-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2897885

Product Specific Information

Positive Samples: A-549, Raji, THP-1, Mouse liver, Mouse spleen, Mouse heart, Rat liver

Immunogen sequence: HRVLGQYLRD TAALSPPKAT VSDTCEEVEP SLLEILPKSS ERTPLPLCSS QAQMDYRRLQ PSCLGTMPLS VCPPMAESGS CCTTHIANHS YLPLSYWQQP

Target Information

In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.