Thermo Fisher Scientific Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Qdot 655
Thermo Fisher의 Donkey anti-Rabbit IgG (H+L) Qdot 655 항체는 토끼 IgG 검출용 형광 2차 항체로, 높은 광안정성과 밝기를 제공합니다. Western blot, IHC, ICC, Flow cytometry 등에 적합하며, 300/654 nm에서 발광합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:500 |
| Immunohistochemistry (IHC) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Qdot™ 655 |
| Excitation / Emission Max | 300 / 654 nm |
| Form | Liquid |
| Concentration | 1 µM |
| Purification | Purified |
| Storage Buffer | 0.05M borate, pH 8.3, with 1M betaine |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient |
| RRID | AB_2556496 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Alternative Formats Available
- Alexa Fluor 488
- Alexa Fluor 594
- AP
- Biotin
- FITC
- HRP
- Qdot 525
- Qdot 565
- Qdot 625
- TRITC
- Custom conjugation available upon request
Product Specific Information
Qdot™ nanocrystals are semiconductor-based fluorescent particles that are exceptionally bright and resistant to photobleaching. When paired with appropriate optical filters, Qdot conjugates can be up to 50 times brighter than traditional organic dyes.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are widely used for detection, sorting, or purification of target proteins.
Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may undergo further purification or modification (e.g., immunoaffinity chromatography, fragmentation, label conjugation).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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