Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine7, eBioscience

Applications

Flow Cytometry (Flow)

• Tested dilution: 0.5 µg/test
• View 80 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 661 / 737
• Brilliant Violet 421 / 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 (~35 kDa) is expressed as a disulfide-linked dimer. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte activation and development.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Flow cytometric analysis of resting and activated mouse splenocytes
• Recommended use: ≤ 0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
• Light sensitivity: Protect from light (photo-induced oxidation sensitive)
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-54)
• Excitation: 488–561 nm
• Emission: 775 nm
• Compatible lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is expressed on activated platelets. CD69 expression is induced upon T lymphocyte activation and may play roles in proliferation and signal transduction in NK cells and platelets.
Diseases associated with CD69 dysfunction include coccidioidomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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