Thermo Fisher Scientific ATF7 Polyclonal Antibody
ATF7 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunoprecipitation에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 1 mg/mL 농도의 액상 형태입니다. Human에 반응하며 4°C에서 보관합니다.
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Applications
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 10–15 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 433 and 483 of human activating transcription factor 7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Orangutan
Target Information
ATF7 plays important functions in early cell signaling. It binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. It acts as an activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Although it has no intrinsic transcriptional activity, it activates transcription upon formation of JUN or FOS heterodimers. It can also bind TRE promoter sequences when heterodimerized with members of the JUN family.
- Isoform 4/ATF-A0 acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.
- Isoform 5/ATF-4 acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities.
- It may exert these effects by sequestering in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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