Thermo Fisher Scientific MUC1/CA15-3/EMA/CD227 Recombinant Rabbit Monoclonal Antibody (MUC1, 1887R)

Applications

Western Blot (WB)

• Tested Dilution: 1–2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Product Specifications

Product Specific Information

Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM, EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic lineages.
It is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through proteolysis and extensive O-linked glycosylation.
MUC1 is highly polymorphic, with alleles encoding variable repeat numbers (30–90), resulting in molecular weight differences.
It is abundantly expressed in >90% of breast carcinomas and metastases.
Transgenic MUC1 associates with all four cebB receptors and localizes with erbB1 (EGFR) in lactating glands.
The MUC1 gene contains seven exons and produces several alternatively spliced variants.
Overexpression, aberrant localization, and altered glycosylation are linked to carcinomas.
Multiple transcript variants encoding different isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.