Thermo Fisher Scientific ENOSF1 Polyclonal Antibody
Thermo Fisher Scientific의 ENOSF1 Polyclonal Antibody는 인간 ENOSF1 단백질을 인식하는 토끼 유래 다클론 항체입니다. Western blot, IHC, ICC/IF에 적합하며 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 13–349 of Human ENOSF1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.1M Tris glycine, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2548949 |
Product Specific Information
- Recommended positive controls: A549, HeLa, HepG2, HCT116
- Predicted reactivity: Pig (88%), Bovine (89%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DNA methylation is a major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins containing a methyl-CpG binding domain (MBD).
Each of these proteins, except MBD3, binds specifically to methylated DNA. MECP2, MBD1, and MBD2 can repress transcription from methylated gene promoters.
Unlike other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensable in stem cells but essential for embryonic development. Mutations in MECP2 cause most cases of Rett syndrome, a progressive neurologic developmental disorder and a common cause of mental retardation in females.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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