Thermo Fisher Scientific von Willebrand Factor/Factor VIII Related-Ag Recombinant Rabbit Monoclonal Antibody (VWF, 1859R)
Recombinant rabbit monoclonal antibody targeting human von Willebrand Factor (VWF). Validated for WB and IHC applications. High purity (Protein A) and concentration (200 µg/mL). Suitable for research use in studying hemostasis mechanisms.
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Applications
- Western Blot (WB): 1–2 µg/mL
- Immunohistochemistry (Paraffin) (IHC (P)): 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | VWF, 1859R |
| Immunogen | Recombinant fragment of human vWF protein (aa1815–1939) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Von Willebrand factor (vWf) consists of self-aggregated glycoprotein subunits synthesized in endothelial cells and megakaryocytes.
The monomeric vWf unit has a molecular mass of 225 kDa and binds to circulating factor VIIIc (antihemophilic factor), stabilizing it and playing a crucial role in normal primary haemostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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