Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI7E8), TrueMAB
UBE2E3 단백질을 표적으로 하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF, Flow cytometry 등에 사용 가능. 인간, 개, 생쥐, 랫트 반응성. 고순도 친화 크로마토그래피 정제, 액상 형태, 연구용 전용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI7E8 |
| Immunogen | Full length human recombinant protein of human UBE2E3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates.
- The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by the Ub-activating enzyme (E1).
- The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex.
- The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the appropriate protein substrate.
UBE2E3 (also designated UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and regulates transepithelial sodium transport in renal cells. It may also be involved in cell growth arrest. The UBE2E3 protein shuttles between the cytoplasm and nucleus in an IPO11-dependent manner and is ubiquitously expressed at low levels, with high expression in skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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