Thermo Fisher Scientific TRAF3 Polyclonal Antibody
TRAF3 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. Protein A로 정제된 액상 형태이며, -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 0.2 µg |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human TRAF3 (amino acids 101–200) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Tumor necrosis factor (TNF)-induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins such as TNF receptor-associated factors (TRAFs).
TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g., RANK, CD30, CD40) and the interleukin-1 receptor.
The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization.
Recent molecular cloning studies have identified six TRAFs (TRAF1–TRAF6). TRAF3, originally named CRAF1, interacts directly with the CD40 cytoplasmic tail through a region similar to TNF receptor-associated factors.
TRAF3 binds only a single site containing the sequence PEQET, whereas TRAF1 and TRAF2 can bind both the PEQET site and an additional downstream domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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