Thermo Scientific™

Thermus thermophilus DNA Ligase

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate자세히 알아보기

Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. Thermus thermophilus DNA Ligase is not active on RNA, single-stranded DNA, or blunt-ended DNA.

Features

• Increased specificity—high ligation temperature eliminates non-specific ligation
• Stabile at high temperatures with an optimum ligation temperature of 45°C to 65°C

Applications

Thermus thermophilus DNA ligase is recommended for applications requiring high ligation specificity, including:

• Single-stranded nick repair in double-stranded DNA
• Next-generation sequencing
• Rolling circle amplification
• Proximity ligation assay

사양

농도50 U/μL

용도(애플리케이션)Single-stranded nick repair, NGS, RCA, PLA

효소DNA Ligase

열 불활성화에 대한 민감성No

수량1000 U

제품 유형NAD-dependent DNA Ligase

연구 카테고리Next-generation Sequencing, DNA Repair

형태Liquid

Unit SizeEach