Thermo Fisher Scientific TNF alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Horse

Published species

Bovine

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Equine TNF alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_417106

Product Specific Information

PETNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Equine samples.

The PETNFAI immunogen is recombinant Equine TNF alpha.

PETNFAI detects TNF Alpha which has a predicted molecular weight of approximately 25 kDa.

The PETNFAI equine TNFa polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with biotinylated detection antibody PETNFABI. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Reconstitute in 1,000 µL PBS to create a stock of 1 mg/mL.

Target Information

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.