Thermo Fisher Scientific DNMT3L Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Store vial at -20°C prior to opening.
Aliquot contents and freeze at -20°C or below for extended storage.
Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid.
Dilute only prior to immediate use.

This antibody is expected to cross-react with mouse, as the human immunogen sequence shows 76% identity with mouse.
Reactivity with DNMT3L from other species has not yet been tested.

Target Information

Methylation of DNA at cytosine residues in mammalian cells is a heritable epigenetic modification essential for gene regulation, genomic imprinting, and development.
Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3.

• DNMT1: Maintenance methyltransferase, expressed in proliferating cells.
• DNMT3A / DNMT3B: De novo methyltransferases, strongly expressed in embryonic stem cells.
• DNMT2: Expressed at low levels in adult tissues; inactivation does not affect methylation.
• DNMT3L: Catalytically inactive regulatory factor for DNMT3A and DNMT3B, expressed in embryonic stem cells, testis, ovaries, and thymus.

DNMT3L forms a heterotetrameric complex with DNMT3A or DNMT3B and contains an N-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a C-terminal methyltransferase-like domain.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.