Thermo Fisher Scientific DNMT3L Polyclonal Antibody
DNMT3L 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ELISA, IP에 사용 가능. 인간 시료에 반응하며, 마우스와도 교차 반응 가능성이 있음. Affinity chromatography로 정제된 액상 항체로, -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 – 1:2,000 |
| Immunohistochemistry (IHC) | 1:500 |
| ELISA | 1:1,000 – 1:3,000 |
| Immunoprecipitation (IP) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region near aa 150–175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.11 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening.
Aliquot contents and freeze at -20°C or below for extended storage.
Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Stable for several weeks at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
This antibody is expected to cross-react with mouse, as the human immunogen sequence shows 76% identity with mouse.
Reactivity with DNMT3L from other species has not yet been tested.
Target Information
Methylation of DNA at cytosine residues in mammalian cells is a heritable epigenetic modification essential for gene regulation, genomic imprinting, and development.
Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3.
- DNMT1: Maintenance methyltransferase, expressed in proliferating cells.
- DNMT3A / DNMT3B: De novo methyltransferases, strongly expressed in embryonic stem cells.
- DNMT2: Expressed at low levels in adult tissues; inactivation does not affect methylation.
- DNMT3L: Catalytically inactive regulatory factor for DNMT3A and DNMT3B, expressed in embryonic stem cells, testis, ovaries, and thymus.
DNMT3L forms a heterotetrameric complex with DNMT3A or DNMT3B and contains an N-terminal ATRX-DNMT3-DNMT3L (ADD) domain and a C-terminal methyltransferase-like domain.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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