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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Rhodamine
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Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Rhodamine

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Rhodamine으로 표지된 염소 유래 anti-Human IgG (H+L) 교차흡착 2차 항체로, IHC, ICC, Flow, IP 등 다양한 응용에 적합합니다. 인간 IgG에 특이적으로 반응하며, 비면역 단백질과의 교차 반응을 최소화했습니다. 형광 검출용으로 높은 신뢰성과 감도를 제공합니다.

카탈로그번호
31683
판매단위
pk
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마지막 업데이트 2025. 08. 03. 오후 10:28
Thermo Fisher Scientific 31683 Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Rhodamine 1.5 mg pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific Goat anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, Rhodamine

Applications and Tested Dilution

Application Dilution
Immunohistochemistry (IHC) 1:50 - 1:200
Immunocytochemistry (ICC/IF) 1:50 - 1:200
Flow Cytometry (Flow) 1:50 - 1:200
Immunoprecipitation (IP) 1:50 - 1:200

Product Specifications

Specification Description
Species Reactivity Human
Host/Isotype Goat / IgG
Class Polyclonal
Type Secondary Antibody
Conjugate Rhodamine
Excitation/Emission Max 573/591 nm
Form Lyophilized
Concentration 1.5 mg/mL
Purification Affinity chromatography
Storage Buffer PBS, pH 7.6, with 15 mg/mL BSA
Contains 0.05% sodium azide
Storage Conditions 4°C
Shipping Conditions Ambient (domestic); Wet ice (international)
RRID AB_429710
Target IgG
Cross Adsorption Against bovine, horse, and mouse serum proteins
Antibody Form Whole Antibody
Country of Origin USA

Additional Formats Available

  • Alexa Fluor 488
  • Alexa Fluor 546
  • Alexa Fluor 555
  • Alexa Fluor 568
  • Alexa Fluor 594
  • Alexa Fluor 633
  • Alexa Fluor 647
  • Alexa Fluor Plus 405
  • Alexa Fluor Plus 488
  • Alexa Fluor Plus 555
  • Alexa Fluor Plus 594
  • Alexa Fluor Plus 647
  • AP
  • Biotin
  • FITC
  • HRP
  • TRITC
  • Custom conjugation available upon request

Product Specific Information

  • Concentration may vary slightly from lot to lot; refer to lot-specific datasheet for exact concentration.
  • Product #31683 has been successfully used in Western blot, IF, ICC, IHC, IP, and FACS applications.
  • Reacts with heavy chains of human IgG and light chains common to most human immunoglobulins, with minimal cross-reactivity to bovine, horse, and mouse serum proteins.
  • Store protected from light at 4°C. For extended storage, add equal volume of glycerol (final 50%) and store at -20°C.
  • Rhodamine Amax = 550 nm; Emax = 570 nm.
  • Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.44 (lot-dependent).
  • Reconstitute with 1.1 mL distilled water (1.5 mg/mL after restoration).

Target Information

Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of target molecules and provide enhanced sensitivity through signal amplification.
Secondary antibodies are typically generated by immunizing host animals with pooled human immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity and performance.


For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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