Thermo Fisher Scientific Human IL-12 Receptor beta-2 (soluble) Fc Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

CKRGDVTVKP SHVILLGSTV NITCSLKPRQ GCFHYSRRNK LILYKFDRRI NFHHGHSLNS QVTGLPLGTT LFVCKLACIN SDEIQICGAE IFVGVAPEQP QNLSCIQKGE QGTVACTWER GRDTHLYTEY TLQLSGPKNL TWQKQCKDIY CDYLDFGINL TPESPESNFT AKVTAVNSLG SSSSLPSTFT FLDIVRPLPP WDIRIKFQKA SVSRCTLYWR DEGLVLLNRL RYRPSNSRLW NMVNVTKAKG RHDLLDLKPF TEYEFQISSK LHLYKGSWSD WSESLRAQTP EEEPTGMLDV WYMKRHIDYS RQQISLFWKN LSVSEARGKI LHYQVTLQEL TGGKAMTQNI TGHTSWTTVI PRTGNWAVAV SAANSKGSSL PTRINIMNLC EAGLLAPRQV SANSEGMDNI LVTWQPPRKD PSAVQEYVVE WRELHPGGDT QVPLNWLRSR PYNVSALISE NIKSYICYEI RVYALSGDQG GCSSILGNSK HKAPLSGPHI NAITEEKGSI LISWNSIPVQ EQMGCLLHYR IYWKERDSNS QPQLCEIPYR VSQNSHPINS LQPRVTYVLW MTALTAAGES SHGNEREFCL QGKANGGPKS CDKTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHHHH

Tag

Fc-tag, His-tag

Molecular weight

94.9 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Human sIL-2 Receptor beta-2 Fc activity is measured by its ability to bind to Human IL-12p70 in a functional ELISA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human sIL-12 Receptor beta-2 Fc has a calculated molecular weight of 94.9 kDa, and contains 839 amino acid residues, which consists of the extracellular domain of IL-12R beta-2 fused to the Fc portion of human IgG and contains a C-terminal His-tag. Due to glycosylation, it migrates at an approximate molecular weight of 116 kDa, under reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn`s disease and leprosy, which is thought to contribute to the inflammatory response and host defense.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.