Thermo Fisher Scientific CD45RO Monoclonal Antibody (T200, 797)
CD45RO 단클론 항체로 인간 시료에 반응하며, IHC, ICC/IF, Flow Cytometry에 적합. Mouse IgG2a, kappa 형식으로 제작된 비결합형 액상 항체. PBS 버퍼에 보존되어 있으며 단백질 A/G로 정제됨. 연구용으로만 사용 가능.
- 카탈로그번호
- 5788-MSM7-P1ABX
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | T200, 797 |
| Immunogen | Human T-lymphocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising approximately 10% of surface proteins in lymphocytes.
It is absent in non-hematopoietic cell lines and tissues. CD45 glycoprotein plays a crucial role in lymphocyte development and antigen signaling, regulating Src-family kinases.
Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing transmembrane and cytoplasmic domains.
CD45RA is one isoform with restricted expression among lymphoid subtypes. CD45 isoforms vary in membrane domain translocation and expression depending on cell type and physiological state.
It is an essential regulator of T- and B-cell receptor signaling and modulates cytokine receptor signaling through JAK kinase suppression.
Additionally, CD45 contributes to cell survival, integrin-mediated signaling, DNA fragmentation during apoptosis, and regulation of various immunological functions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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