Thermo Fisher Scientific eIF5B/IF2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Orangutan

Target Information

Accurate initiation of translation in eukaryotes requires multiple initiation factors. In prokaryotes, only three factors (IF1, IF2, IF3) are involved. Two of these are conserved in eukaryotes: eIF1A (homolog of IF1) and eIF5B (homolog of IF2). The eIF5B gene encodes a protein that interacts with eIF1A and other initiation factors on the ribosome, along with GTP, to correctly position the initiator methionine tRNA at the start codon of mRNA, ensuring accurate translation initiation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.